RNA

Part:BBa_K3406016

Designed by: Xiaojie Zhou   Group: iGEM20_ZJUT_China_B   (2020-10-27)


LbaCas13a-MERS-CoV-crRNA

The crRNA is an oligonucleotide chain, it can combine with Cas13 protein, and guide it to find the target viral sequences. It consists of two parts, DR sequence and spacer. The DR sequence is determined by Cas protein, and different Cas proteins have different DR sequences. And the spacer is determined by a specific target. Here the crRNA is corresponding to LbaCas13a and MESR-CoV, which could combine with LbaCas13a protein and guide it to target MERS-CoV sequence, thus activating the collateral cleavage of LbaCas13a protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 49
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 9


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Categories
Parameters
None